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IVF: (In vitro Fertilization)

In vitro fertilization involves both the female and the male partners.  Eggs are retrieved from the ovaries and are placed with the male partner’s sperm in a petri dish in a laboratory, where fertilization occurs to produce embryos.  After the embryos have matured for several days in the laboratory, they are placed into the uterus for implantation and a pregnancy should result.

At the proper time in her cycle, the female partner is given hormone medication that should produce multiple eggs in each ovary.  Egg production is monitored by a series of vaginal ultrasounds and blood tests.  When the eggs are ready, the patient is sedated in the office, and the eggs are retrieved using an ultrasound guided needle through the vagina.  The needle is advanced into the ovarian follicle and the eggs are aspirated into a test tube for transport to the lab. After the retrieval procedure, the patient spends a short time recovering and can then go home.  Limited activity is recommended for a time.

Just prior to egg retrieval, the male partner’s sperm is obtained and processed.  A suspension of the best population of sperm is added to the eggs for fertilization.  If the quality of the sperm is very poor, ICSI (intracytoplasmic sperm injection) can be performed.  When ICSI is used, a single sperm is captured in a needle-like pipet and is then injected directly into the egg to facilitate fertilization.

The eggs and sperm are monitored regularly to assess fertilization and the production of embryos.  Embryos are allowed to mature for several days until they reach the blastocyst stage.

When the embryos are ready, 2 or 3 are transferred into the uterus using a small catheter threaded through the cervix.  No sedation is required; the transfer takes approximately 10 minutes.  After a short rest period, the patient can go home.  Again, limited activity is recommended for a time. Any remaining embryos can be cryopreserved, or frozen, for possible use with another cycle. 

 

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Intrauterine Insemination

Intrauterine Insemination is an office procedure where washed sperm are delivered directly into the uterus using a small catheter that is passed through the cervix.  The procedure is relatively quick with very minimal discomfort.  The cervical mucus, which can sometimes be hostile to the sperm, is completely bypassed.  All of the sperm in the ejaculation are placed in the uterus as compared to the 1% (approximately) that make it during natural intercourse.

 

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Artificial Insemination with Donor Sperm

Success in achieving a pregnancy depends on the quality of both the female egg and the male sperm. In some cases, treatment for male factor infertility is unsuccessful. Donor sperm from an approved sperm bank may be used in conjunction with intrauterine insemination (IUI). The sperm bank provides profiles on various proven sperm donors and a donor can be selected based upon his genetic and physical factors.

 

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Ovulation Induction:

Occasionally, a woman’s body does not produce mature eggs on its own during a monthly cycle.  It may be necessary to use medication to help stimulate the maturation of one or more eggs.  This is referred to as ovulation induction.  Ovulation induction may be achieved by taking pills for several days during a cycle or may require alternate medication in the form of injections. 

 

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Hysteroscopic Evaluation of the Uterus

The uterine cavity can be visualized by inserting a small optical tube called a hysteroscope, through the vagina and cervix. The uterus is filled with saline to enhance visualization.  The physician can evaluate the size and depth of the uterus, the presence of abnormalities inside the uterus, the presence of polyps or adhesions, and the quality of the endometrium.  The procedure is usually performed in the early half of a woman’s cycle and can be done in the office.  It takes approximately 20 minutes.

 

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Hysterosalpingogram:

A hysterosalpingogram (HSG) is a procedure used to x-ray the uterine cavity and fallopian tubes.  It is performed in the x-ray department and takes approximately 15 minutes. The patient is allowed to go home after the procedure.  A thin catheter is passed through the cervix into the uterus. The uterine cavity and fallopian tubes are then flushed with an x-ray contrast dye (through the catheter).  There may be some cramping associated with the injection of the dye. The movement of the dye is observed as it makes its way through the uterus into the fallopian tubes and spills into the pelvic area.  An HSG can detect: an abnormally shaped uterine cavity, fibroid tumors, polyps, scar tissue, partially or completely blocked fallopian tubes, and normally shaped uterine cavity and tubes.

 

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Tubal Catheterization:

When a blocked fallopian tube is discovered with a hysterosalpingogram (HSG), a tubal catheterization can be performed to correct the blockage.  The procedure is performed in the x-ray department and takes approximately 20 minutes.  The patient can go home after the procedure.  Under fluoroscopic guidance, a thin balloon catheter is threaded through the cervix, uterus and into the affected fallopian tube.  At the site of the blockage, the balloon is inflated, correcting the obstruction.  The area is then flushed with an x-ray contrast dye to verify results.  There is an 85 – 90% chance that a blocked tube will be opened.  About 60% of those will still be open after 6 months, and 30 – 40 % will conceive.

 

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Follicular Cyst Aspiration

Typically, ovarian cysts are not disease related and disappear on their own.  During ovulation, a follicle grows but fails to rupture and release an egg.  It retains fluid and becomes a cyst.  Ovarian cysts can cause a variety of symptoms including abnormal uterine bleeding, pelvic pain, and nausea and vomiting.  An ovarian cyst can be drained in the office using a needle with ultrasound guidance.  The procedure takes approximately 15 minutes.

 

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Embryo Cryopreservation:

Often, an IVF cycle will result in more embryos than can be used for that cycle.  Extra embryos can be cryopreserved, or frozen, in liquid nitrogen at an extremely low temperature.  They can then be saved for possible use in another otherwise natural cycle, sparing the patient from undergoing ovulation induction, egg retrieval, etc.  Approximately 50% of frozen embryos will survive the thawing process.

 

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Dr. Barry Witten performing reproductive services including in vitro fertilization, intracytoplasmic sperm injection, embryo cryopreservation, sperm cryopreservation, ovulation induction, intrauterine insemination, post-coital test, follicle study ultrasound, follicular cyst aspiration, endometrial biopsy, cultures, reversal of tubal ligation, exploratory laparotomy, diagnostic laparoscopy, laser vaporization of endometriosis, laser vaporization of pelvic adhesions for St. Louis and the surrounding area.

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